Coding

Part:BBa_K5301008:Design

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-22)


MCherry [1-10] is a part of bimolecular fluorescence complementation (BiFC) system.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be codon-optimized for expression in the certain chassis.

Source

It was developed by splitting mCherry, a mutant monomeric red fluorescent protein, into two fragments. And that sequence is from Bo Huang's lab.

References